General Instruction: Trifectant is effective against numerous microorganisms affecting animals; viruses, gram positive and gram negative bacteria, fungi (molds and yeasts), and mycoplasma. Efficacy was determined in the presence of hard water and organic material. Trifectant passes the AOAC germicidal and detergent sanitizer test and a concentration of 0.5% (1:200) in the presence of 200 PPM hard water. Apply a 0.5% solution for routine sanitation. Remove all animals and their food from the premises, trucks or other vehicles, coops, crates or other enclosures.